basic_info |
|
| Product_Name： | 蛋白酶 K |
| CAS： | 39450-01-6 ;9001-92-7 |
| 中文同义词： | 蛋白激酶K ; 蛋白酶K 来源于林伯氏白色念球菌(溶液) ; 蛋白酶K 来源于林伯氏白色念球菌 ; 蛋白酶 K ; 蛋白酶K |
| EnglishSynonyms： | IUB: 3.4.21.64 ; ENDOPEPTIDASE K ; TRITIRACHIUM ALBUM ; PROTEASE ISOLATED FROM S. AUREUS STRAIN V8 ; PROTEINASE K FROM TRITIRACHIUM ALBUM LIMBER ; PROTEASE ALKALINE FROMSTREPTOMYCES GRISEUS ; PROK ; PROTEASE FROM STREPTOMYCES CAESPITOSUS SUBSTANTIALLY FREE OF DNASE II AND RNASE. ; TRITIRACHIUM ALKALINE PROTEINASE ; PROTEINASE K, TRITIRACHIUM ALBUM LIMBER ; 3.4.21.14 ; EC 3.4.21.64 ; PROTEINASE K FROM TRITIRACHIUM ALBUM LIMBER(SOLUTION) ; EC 3.4.21.14 ; PROTEINASE K ; PROTEASE FUNGAL FROM RHIZOPUS SPECIES ; PROTEASE FROM BACILLUS THERMOPROTEOLYTICUS ROKKO TYPE X ; PROTEASE FUNGAL FROM ASPERGILLUS SAITOI SUBSTANTIALLY FREE OF ALKALINE PROTEASE ACTIVITY: APPROX. 0.3 UNIT MG SOLID ; IUB: 3.4.21.14 |
| pro_mdlNumber： | MFCD00132129 |
| pro_acceptors： | 0 |
| pro_donors： | 0 |
| InChi： | InChI=1S// |
| InChiKey： | InChIKey=MOSFIJXAXDLOML-UHFFFAOYSA-N |
property |
|
| Comments： | APPLICATION: USEFUL FOR THE PROTEOLYTIC INACTIVATION OF NUCLEASES DURING THE ISOLATION OF DNA AND RNA. REMOVAL ENDOTOXINS BOUND TO CATIONIC PROTEINS SUCH AS LYSOZYME AND RIBONUCLEASE A.REPORTED USEFUL FOR THE ISOLATION OF HEPATIC, YEAST, AND MUNG BEAN MITOCHONDRIADETERMINATION OF ENZYME LOCALIZATION ON MEMBRANESTREATMENT OF PARAFFIN EMBEDDED TISSUE SECTIONS TO EXPOSE ANTIGEN BINDING SITES FOR ANTIBODY LABELING.DIGESTION OF PROTEINS FROM BRAIN TISSUE SAMPLES FOR PRIONS IN TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (TSE) RESEARCH BIOCHEM/PHYSIOL ACTIONS: PROTEINASE K IS A STABLE AND HIGHLY REACTIVE SERINE PROTEASE. EVIDENCE FROM CRYSTAL AND MOLECULAR STRUCTURE STUDIES INDICATES THE ENZYME BELONGS TO THE SUBTILISIN FAMILY WITH AN ACTIVE-SITE CATALYTIC TRIAD (ASP39-HIS69-SER224). IT IS STABLE IN A BROAD RANGE OF ENVIRONMENTS: PH, BUFFER SALTS, DETERGENTS (SDS), AND TEMPERATURE. IN THE PRESENCE OF 0.1-0.5% SDS, PROTEINASE K RETAINS ACTIVITY AND WILL DIGEST A VARIETY OF PROTEINS AND NUCLEASES IN DNA PREPARATIONS WITHOUT COMPROMISING THE INTEGRITY OF THE ISOLATED DNA COLOR: WHITE FORM: POWDER STORAGE TEMPERATURE: 2-8 DEG C UNIT DEFINITION: 1 U CORRESPONDS TO THE AMOUNT OF ENZYME WHICH HYDROLYZES 1 MICROMOLE N-ACETYL-L-TYROSINE-ETHYL ESTER (ATEE) PER MINUTE AT PH 9.0 AND 30 DEG C UNSPSC: 12352200 WGK: 1 |
| Information： | BIOLOGICAL SOURCE: FROM TRITIRACHIUM ALBUM MW: 28.93 KDA |
| Concentration： | ≥10mg/mL |
secure_info |
|
| secure_symbol： |
GHS07
GHS08
|
| secure_signal_word： | Danger |
| secure_risk_stmt： | H315-H319-H334-H335 |
| secure_cautionary_stmt： | P261-P305 + P351 + P338-P342 + P311 |
| secure_damage_code： | Xn |
| secure_risk_disclosure_stmt： | R:36/37/38-42 |
| secure_security_stmt： | S:22-24-26-36/37 |
| secure_wgk_germany： | 1 |
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